Difference between revisions of "Part:BBa K515100"

Revision as of 13:20, 12 September 2011

IAA biosynthetic genes under control of the Pveg2 promoter

The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH). There are several different pathways that produce indole-3-acetic acid. [1] We are expressing genes of the IAM pathway that originate from Psuedomonas savastanoi in E. coli. IaaM and IaaH have been expressed in E. coli previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 547
Illegal BamHI site found at 1492
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 254
Illegal NgoMIV site found at 2835
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K515100 short</partinfo>
-The construct of IaaM and IaaH genes in PVEG plasmid
+<p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH). There are several different pathways that produce indole-3-acetic acid. [1] We are expressing genes of the IAM pathway that originate from Psuedomonas savastanoi in E. coli. IaaM and IaaH have been expressed in <i>E. coli</i> previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. </p>
 <!-- Add more about the biology of this part here