Difference between revisions of "Part:BBa K592008:Design"

 
(→‎Design Notes)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K592003 short</partinfo>
+
<partinfo>BBa_K592008 short</partinfo>
  
−
<partinfo>BBa_K592003 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K592008 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.  
+
The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed, slowing bacterial growth. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 22:02, 11 September 2011

T5-lac Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed, slowing bacterial growth. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.

Source

Phage T5

References