Difference between revisions of "Part:BBa K515002"
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<partinfo>BBa_K515002 short</partinfo> | <partinfo>BBa_K515002 short</partinfo> | ||
<p><b>Description</b></p> | <p><b>Description</b></p> | ||
− | <p>Part of biobrick <a href="parts.igem.org/Part:BBa_K515102"> BBa_K515102</a>, includes optimised insulator, RBS and coding sequence for malate responsive chemoreceptor PA2652 from <i>Pseudomonas aeruginosa PA01</i>. Insulator allows the strength of the RBS to remain the same even when the promoters for this biobrick are changed using PCR, with insulator as start for primers.The translation initiation rate for the RBS sequence is 42010.</p> | + | <p>Part of biobrick <a href="https://parts.igem.org/Part:BBa_K515102"> BBa_K515102</a>, includes optimised insulator, RBS and coding sequence for malate responsive chemoreceptor PA2652 from <i>Pseudomonas aeruginosa PA01</i>. Insulator allows the strength of the RBS to remain the same even when the promoters for this biobrick are changed using PCR, with insulator as start for primers.The translation initiation rate for the RBS sequence is 42010.</p> |
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Revision as of 13:06, 11 September 2011
PA2652
Description
Part of biobrick <a href="https://parts.igem.org/Part:BBa_K515102"> BBa_K515102</a>, includes optimised insulator, RBS and coding sequence for malate responsive chemoreceptor PA2652 from Pseudomonas aeruginosa PA01. Insulator allows the strength of the RBS to remain the same even when the promoters for this biobrick are changed using PCR, with insulator as start for primers.The translation initiation rate for the RBS sequence is 42010.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 675
Illegal NgoMIV site found at 777
Illegal AgeI site found at 83 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 984