Difference between revisions of "Part:BBa K525560:Design"
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+ | Sasaki M, Akahira A, Oshiman K, Tsuchido T, Matsumura Y (2005) Purification of Cytochrome P450 and Ferredoxin, Involved in Bisphenol A Degradation, from ''Sphingomonas'' sp. Strain AO1, [http://aem.asm.org/cgi/content/abstract/71/12/8024 ''Appl Environ Microbiol'' 71(12):8024-8030]. | ||
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+ | Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in ''Sphingomonas bisphenolicum'' strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full ''J Appl Microbiol'' 105(4):1158-1169]. |
Revision as of 09:10, 11 September 2011
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1350
Illegal BamHI site found at 2088 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 2341 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 685
Design Notes
Assembled
Source
- BBa_K525499: NADP+ ferredoxin oxidoreductase from Escherichia coli TOP10
- BBa_K123000: bisdA gene from Sphingomonas bisphenolicum AO1, probably synthesized and codon optimized (see experience).
- BBa_K123000: bisdB gene from S. bisphenolicum AO1, probably synthesized and codon optimized (see experience).
References
Sasaki M, Akahira A, Oshiman K, Tsuchido T, Matsumura Y (2005) Purification of Cytochrome P450 and Ferredoxin, Involved in Bisphenol A Degradation, from Sphingomonas sp. Strain AO1, [http://aem.asm.org/cgi/content/abstract/71/12/8024 Appl Environ Microbiol 71(12):8024-8030].
Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].