Difference between revisions of "Part:BBa K525560:Design"
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* <partinfo>BBa_K525499</partinfo>: NADP<sup>+</sup> ferredoxin oxidoreductase from ''Escherichia coli'' TOP10 | * <partinfo>BBa_K525499</partinfo>: NADP<sup>+</sup> ferredoxin oxidoreductase from ''Escherichia coli'' TOP10 | ||
| − | * <partinfo>BBa_K123000</partinfo>: ''bisdA'' gene from ''Sphingomonas bisphenolicum'' AO1, probably synthesized and codon optimized (see [https://parts.igem.org/Part:BBa_K123000:Experience experience). | + | * <partinfo>BBa_K123000</partinfo>: ''bisdA'' gene from ''Sphingomonas bisphenolicum'' AO1, probably synthesized and codon optimized (see [https://parts.igem.org/Part:BBa_K123000:Experience experience]). |
| − | * <partinfo>BBa_K123000</partinfo>: ''bisdB'' gene from ''S. bisphenolicum'' AO1, probably synthesized and codon optimized (see [https://parts.igem.org/Part:BBa_K123001:Experience experience). | + | * <partinfo>BBa_K123000</partinfo>: ''bisdB'' gene from ''S. bisphenolicum'' AO1, probably synthesized and codon optimized (see [https://parts.igem.org/Part:BBa_K123001:Experience experience]). |
===References=== | ===References=== | ||
Revision as of 09:09, 11 September 2011
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1350
Illegal BamHI site found at 2088 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 2341 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 685
Design Notes
Assembled
Source
- BBa_K525499: NADP+ ferredoxin oxidoreductase from Escherichia coli TOP10
- BBa_K123000: bisdA gene from Sphingomonas bisphenolicum AO1, probably synthesized and codon optimized (see experience).
- BBa_K123000: bisdB gene from S. bisphenolicum AO1, probably synthesized and codon optimized (see experience).