Difference between revisions of "Part:BBa K515000"
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IaaM encodes IAA tryptophan monooxygenase which converts tryptophan to indoleacetamide. The sequence for this is from <i>Psuedomonas savastanoi</i> which uses a two-enzyme pathway to produce indole 3 acetic acid (auxin).  
 
IaaM encodes IAA tryptophan monooxygenase which converts tryptophan to indoleacetamide. The sequence for this is from <i>Psuedomonas savastanoi</i> which uses a two-enzyme pathway to produce indole 3 acetic acid (auxin).  
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The sequence has been codon optimised for <i>E. coli</i> and <i>B. subtilis</i> and is under control of the pVEG promoter with an RBS also suitable for <i>E. coli</i> and <i>B. subtilis</i>.
  
 
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Revision as of 11:24, 9 September 2011

IaaM - tryptophan-2-mono-oxygenase

IaaM encodes IAA tryptophan monooxygenase which converts tryptophan to indoleacetamide. The sequence for this is from Psuedomonas savastanoi which uses a two-enzyme pathway to produce indole 3 acetic acid (auxin).

The sequence has been codon optimised for E. coli and B. subtilis and is under control of the pVEG promoter with an RBS also suitable for E. coli and B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]