Difference between revisions of "Part:BBa J33203:Experience"
(→Applications of BBa_J33203) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_J33203=== | ===Applications of BBa_J33203=== | ||
| + | |||
| + | In 2010, Team Debrecen-Hungary has decided to give a hand with testing the BBa_J33203 part. As the South-East of Hungary is highly affected by the arsenic problem (there are wells in which the level of arsenic is at least 15-fold higher than the WHO limit of 10 ug/l), we had the possibility to use real-world samples for testing. | ||
| + | |||
| + | We collected samples from two wells which were known to contain elevated arsenic concentration. We used DH5 alpha E. coli cell line with lacZDM15 mutation as host cells, than we prepared a medium consisting of ''peptone, yeast extract, NaCl, K2HPO4, NaHCO3, bromthymol blue, and autoclaved water samples of different dilutions''. We spiked these media with the transformed cells, and after 24 hours of incubation at 37°C we received that the part works also on real world samples, not only on samples made of water and sodium-arsenate. The colour change of Bromothymol Blue seemed to be directly proportional to the concentration of arsenic. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:45, 8 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J33203
In 2010, Team Debrecen-Hungary has decided to give a hand with testing the BBa_J33203 part. As the South-East of Hungary is highly affected by the arsenic problem (there are wells in which the level of arsenic is at least 15-fold higher than the WHO limit of 10 ug/l), we had the possibility to use real-world samples for testing.
We collected samples from two wells which were known to contain elevated arsenic concentration. We used DH5 alpha E. coli cell line with lacZDM15 mutation as host cells, than we prepared a medium consisting of peptone, yeast extract, NaCl, K2HPO4, NaHCO3, bromthymol blue, and autoclaved water samples of different dilutions. We spiked these media with the transformed cells, and after 24 hours of incubation at 37°C we received that the part works also on real world samples, not only on samples made of water and sodium-arsenate. The colour change of Bromothymol Blue seemed to be directly proportional to the concentration of arsenic.
User Reviews
UNIQd8df85843b3cff6a-partinfo-00000000-QINU UNIQd8df85843b3cff6a-partinfo-00000001-QINU