Difference between revisions of "Part:BBa K626000"
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The minimal CMV promoter ensures a continuous expression of the protein, but furthermore it can be expressed in an inducible form using the Tetracyclin-controlled transcriptional activation system. | The minimal CMV promoter ensures a continuous expression of the protein, but furthermore it can be expressed in an inducible form using the Tetracyclin-controlled transcriptional activation system. | ||
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+ | hBAX | ||
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+ | Bax (Bcl-2-associated X) protein is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis through cytochrome c release which is the most frequent type of PCD (Programmed cell death). | ||
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Revision as of 14:59, 1 September 2011
TRE-Bax-PolyA in PSB1A3 (expression vector)
Expression vector from standard BioBrick parts, expressing human pro-apoptotic protein BAX.
The human BAX is a pro-apoptotic protein which can be expressed by this expression vector.
The minimal CMV promoter ensures a continuous expression of the protein, but furthermore it can be expressed in an inducible form using the Tetracyclin-controlled transcriptional activation system.
hBAX
Bax (Bcl-2-associated X) protein is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis through cytochrome c release which is the most frequent type of PCD (Programmed cell death).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 778
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]