Difference between revisions of "Part:BBa K639002:Design"

 
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===Design Notes===
 
===Design Notes===
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The BioBrick that the Berkeley team made is in BBb format. This means that the BioBrick is flanked by a prefix and a suffix that is different from the ones that are normally used. Therefore, this BioBrick was initially not compatible with the other BioBricks of normal standard (BBa). To deal with this problem, the rrnB P1 promoter was amplified using PCR. The original promoter made by the Berkeley iGEM team was used as template DNA. To make the amplified brick compatible with the rest of our construct, the standard prefix and suffix was added to our primers.
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The similar BioBrick (BBa_K112118) that the Berkeley team made in 2008 is in BBb format. This means that the BioBrick is flanked by a prefix and a suffix that is different from the standard ones. This BioBrick are therefore not compatible with the other BioBricks of standard format (BBa). To deal with this problem, the rrnB P1 promoter was amplified using PCR. The original promoter made by the Berkeley iGEM team was used as template DNA. To make the amplified brick compatible with the standard format, the standard prefix and suffix was added to our primers. The 11 first base pairs were lost due to difficulties in primer design, but we do not believe this effects the promoter.
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===Source===
 
===Source===

Revision as of 08:35, 26 August 2011

rrnB P1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The similar BioBrick (BBa_K112118) that the Berkeley team made in 2008 is in BBb format. This means that the BioBrick is flanked by a prefix and a suffix that is different from the standard ones. This BioBrick are therefore not compatible with the other BioBricks of standard format (BBa). To deal with this problem, the rrnB P1 promoter was amplified using PCR. The original promoter made by the Berkeley iGEM team was used as template DNA. To make the amplified brick compatible with the standard format, the standard prefix and suffix was added to our primers. The 11 first base pairs were lost due to difficulties in primer design, but we do not believe this effects the promoter.

Source

BBa_K112118, rrnB P1 promoter BioBrick in BBb format made by the Berkley iGEM team in 2008.

References