Difference between revisions of "Part:BBa K091104:Experience"
(→User Reviews) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 18: | Line 17: | ||
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
| + | {|width='80%' style='border:1px solid gray' | ||
| + | |- | ||
| + | |width='10%'| | ||
| + | <partinfo>BBa_K091104 AddReview number</partinfo> | ||
| + | <I>SarahV</I> | ||
| + | |width='60%' valign='top'| | ||
| + | We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. | ||
| + | However, we see a fragment of 1000bp and of ±2000bp. | ||
| + | |} | ||
| + | [[Image:Restriction_1807.jpg]] | ||
| + | |||
<!-- DON'T DELETE --><partinfo>BBa_K091104 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K091104 EndReviews</partinfo> | ||
Revision as of 11:23, 9 August 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K091104
User Reviews
UNIQ0b58b158e8be0b2c-partinfo-00000000-QINU
|
No review score entered. SarahV |
We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and of ±2000bp. |
UNIQ0b58b158e8be0b2c-partinfo-00000002-QINU