Difference between revisions of "Part:BBa K652000"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K652000 short</partinfo>
 
<partinfo>BBa_K652000 short</partinfo>
  
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This part contains a T7 strong promoter, RBS, and our eGFP-fusion protein containing the Rhagium inquistor antifreeze protein (RiAFP) with a His tag at the C-terminus.
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This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Rhagium inquistor antifreeze protein (RiAFP) with a His tag at the C-terminus, followed by a terminator.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:38, 5 August 2011

T7-RBS-eGFP-TEV-RiAFP-His-Term

This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Rhagium inquistor antifreeze protein (RiAFP) with a His tag at the C-terminus, followed by a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 894
    Illegal AgeI site found at 990
    Illegal AgeI site found at 1104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 74
    Illegal SapI.rc site found at 87