Difference between revisions of "Part:BBa K606020:Design"
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===References=== | ===References=== | ||
+ | Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997<br> | ||
+ | Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004 |
Latest revision as of 14:01, 27 July 2011
GFP:LacI protein fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 733
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Design Notes
2 Quick Change Mutagenesis on the basic sequence has been done
Source
Andrew Wright, Dave Lane, pSG20 plasmid.
References
Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997
Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004