Difference between revisions of "Part:BBa K523001"
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===Usage and Biology===
 
===Usage and Biology===
  
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The product protein is believed to be periplasmic and thus may not actually be capable of degrading starch, unless it leaks from the periplasm in significant quantities.
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The product protein is believed to be periplasmic and thus may not actually be capable of degrading starch, unless it leaks from the periplasm in significant quantities. Its natural function in ''E. coli'' presumably involves degrading shorter glucose chains.
  
  

Revision as of 15:23, 19 July 2011

RBS + malS (E. coli periplasmic α-amylase)

This is the E. coli Amylase gene malS. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

Usage and Biology

The product protein is believed to be periplasmic and thus may not actually be capable of degrading starch, unless it leaks from the periplasm in significant quantities. Its natural function in E. coli presumably involves degrading shorter glucose chains.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
  • 1000
    COMPATIBLE WITH RFC[1000]