Difference between revisions of "Part:BBa K523001"
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<partinfo>BBa_K523001 short</partinfo> | <partinfo>BBa_K523001 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The product protein is believed to be periplasmic and thus may not actually be capable of degrading starch, unless it leaks from the periplasm in significant quantities. | ||
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Revision as of 14:48, 19 July 2011
RBS + malS (E. coli periplasmic α-amylase)
This is the E. coli Amylase gene malS. The part contains the native Ribosome Binding Site.
The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1233
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 369
Illegal AgeI site found at 1534 - 1000COMPATIBLE WITH RFC[1000]