Difference between revisions of "Part:BBa K124017:Experience"
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Sequencing by the 2011 iGEM Team Freiburg supports the finding of the Caltech team 2011. Additionally following errors compared to <partinfo>K124014</partinfo> were found: | Sequencing by the 2011 iGEM Team Freiburg supports the finding of the Caltech team 2011. Additionally following errors compared to <partinfo>K124014</partinfo> were found: | ||
| − | *The wrong prefix was used what will finally lead to a RBS- | + | *The wrong prefix was used what will finally lead to a RBS-ATG distance of 8 instead of 6 nucleotides. |
*Two single nucleotide exchanges could be found. | *Two single nucleotide exchanges could be found. | ||
Latest revision as of 08:58, 14 July 2011
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K124017
User Reviews
Caltech 2010: It appears that this part's sequence has been mixed up with BBa_K124014. The gel data on each part page appears to corroborate this mix-up.
Sequencing by the 2011 iGEM Team Freiburg supports the finding of the Caltech team 2011. Additionally following errors compared to BBa_K124014 were found:
- The wrong prefix was used what will finally lead to a RBS-ATG distance of 8 instead of 6 nucleotides.
- Two single nucleotide exchanges could be found.
- The overall sequence resembles the wildtype phage sequence and not the artificially constructed sequence submitted for BBa_K124014.
UNIQ472bf3e0d203122b-partinfo-00000003-QINU UNIQ472bf3e0d203122b-partinfo-00000004-QINU