Difference between revisions of "Part:BBa K274004:Experience"

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E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.
 
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E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.
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Karina Arnesen, undergraduate, Alberta iGEM 2010
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When digested with BamHI and NotI, the insert did not digest as expected, only a 2kb band (backbone) and ~6kb band were visible.

Revision as of 21:44, 28 June 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K274004

User Reviews

UNIQ76771d55c68105bd-partinfo-00000000-QINU UNIQ76771d55c68105bd-partinfo-00000001-QINU

E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.


Karina Arnesen, undergraduate, Alberta iGEM 2010

When digested with BamHI and NotI, the insert did not digest as expected, only a 2kb band (backbone) and ~6kb band were visible.