Difference between revisions of "Part:BBa K523000:Design"
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===Source=== | ===Source=== | ||
− | Based on part <partinfo>BBa_J33207</partinfo>, which was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). | + | Based on part <partinfo>BBa_J33207</partinfo>, which was amplified from ''E. coli'' BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). |
===References=== | ===References=== |
Revision as of 21:22, 28 June 2011
Plac + lacZ; with BglII site
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Note that the BglII (agatct) site overlaps the BioBrick prefix. Since the prefix ends "ag", only "atct" is included in the BioBrick proper.
Source
Based on part BBa_J33207, which was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).