Difference between revisions of "Part:BBa M36699:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This is an updated version of BBa_M36707 | + | This is an updated version of BBa_M36707. The reason we had to alter the part was because of a missing part of the promoter. In the M36707 version, the -10 region of the promoter was missing, as well as a sequence of what we believe is spacing DNA. When using multiple sources to figure out the sequence we needed, there was a mis-numbering in which the full promoter was included on the one website (biocyc.org) while the numbering on the website we actually copied the sequence from, (genome.jp) had a slightly different numbering system. Without the -10 region of the promoter, and the spacer following it, not only would the polymerase not bind, but without the geographic spacing between the binding site and the start codon, there may not be adequate space for the gene downstream to be synthesized. Thus we concluded that without the -10 region of the promoter, the polymerase will never bind and thus the downstream gene will never made even in the absence of IclR and pyruvate. As for without the spacer, even if the polymerase can bind the DNA, the downstream gene still will not be made because the ribosome does not have adequate space to bind and find the start codon. |
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===Source=== | ===Source=== | ||
Revision as of 21:23, 6 May 2011
PaceBAK
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 207
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is an updated version of BBa_M36707. The reason we had to alter the part was because of a missing part of the promoter. In the M36707 version, the -10 region of the promoter was missing, as well as a sequence of what we believe is spacing DNA. When using multiple sources to figure out the sequence we needed, there was a mis-numbering in which the full promoter was included on the one website (biocyc.org) while the numbering on the website we actually copied the sequence from, (genome.jp) had a slightly different numbering system. Without the -10 region of the promoter, and the spacer following it, not only would the polymerase not bind, but without the geographic spacing between the binding site and the start codon, there may not be adequate space for the gene downstream to be synthesized. Thus we concluded that without the -10 region of the promoter, the polymerase will never bind and thus the downstream gene will never made even in the absence of IclR and pyruvate. As for without the spacer, even if the polymerase can bind the DNA, the downstream gene still will not be made because the ribosome does not have adequate space to bind and find the start codon.
Source
See BBa_M36707