Difference between revisions of "Part:BBa M36701"

Latest revision as of 22:22, 5 May 2011

Pyruvate Sensor with Gemini Actuator

IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter into the same plasmid, the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it. While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher. We choose to order to pieces of DNA to test the influence of signal leveling between the frequency of the IclR gene and aceBAK promoter. In this composite, the IclR gene is occurring in an equal frequency as the aceBAK promoter. See the Part Design for more information accounting for the ability of IclR to inhibit the aceBAK promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 679
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2022

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_M36701 short</partinfo>
-Constitutive promoter, RBS, IclR gene, terminator, aceBAK promoter, RBS, Gemini, terminator.  This is designed so that the IclR gene is synthesized on the same scale as the aceBAK promoter instead of relying on IclR naturally in E. coli.
+IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter into the same plasmid, the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it. While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher. We choose to order to pieces of DNA to test the influence of signal leveling between the frequency of the IclR gene and aceBAK promoter. In this composite, the IclR gene is occurring in an equal frequency as the aceBAK promoter.
+See the Part Design for more information accounting for the ability of IclR to inhibit the aceBAK promoter.
 <!-- Add more about the biology of this part here