Difference between revisions of "Part:BBa M36706"
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IclR represses the aceBAK promoter in E. coli.  In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited.  The sequence for the IclR gene was found using genome.jp  The IclR gene is highlighted here in blue: http://www.genome.jp/kegg-bin/cut_sequence_genes.pl?FROM=5092368&TO=5094223&VECTOR=1&ORG=ece&CHR=cx
 
IclR represses the aceBAK promoter in E. coli.  In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited.  The sequence for the IclR gene was found using genome.jp  The IclR gene is highlighted here in blue: http://www.genome.jp/kegg-bin/cut_sequence_genes.pl?FROM=5092368&TO=5094223&VECTOR=1&ORG=ece&CHR=cx
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Since IclR is naturally made in E. coli, we did not need to do any codon optimization, and the start and stop codons were already included.
 
  
  

Revision as of 21:11, 5 May 2011

Gene encoding IclR

IclR represses the aceBAK promoter in E. coli. In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited. The sequence for the IclR gene was found using genome.jp The IclR gene is highlighted here in blue: http://www.genome.jp/kegg-bin/cut_sequence_genes.pl?FROM=5092368&TO=5094223&VECTOR=1&ORG=ece&CHR=cx


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 597
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]