Difference between revisions of "Part:BBa M36706"
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<partinfo>BBa_M36706 short</partinfo> | <partinfo>BBa_M36706 short</partinfo> | ||
− | IclR represses the aceBAK promoter in E. coli. In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited. The sequence for the IclR gene was found using genome.jp The IclR gene is highlighted here in blue: http://www.genome.jp/kegg-bin/cut_sequence_genes.pl?FROM=5092368&TO=5094223&VECTOR=1&ORG=ece&CHR= | + | IclR represses the aceBAK promoter in E. coli. In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited. The sequence for the IclR gene was found using genome.jp The IclR gene is highlighted here in blue: http://www.genome.jp/kegg-bin/cut_sequence_genes.pl?FROM=5092368&TO=5094223&VECTOR=1&ORG=ece&CHR=cx |
+ | |||
+ | Since IclR is naturally made in E. coli, we did not need to do any codon optimization, and the start and stop codons were already included. | ||
Revision as of 20:56, 5 May 2011
Gene encoding IclR
IclR represses the aceBAK promoter in E. coli. In the presence of pyruvate, IclR binds the aceBAK promoter regions more tightly and thus the downstream genes are highly inhibited. The sequence for the IclR gene was found using genome.jp The IclR gene is highlighted here in blue: http://www.genome.jp/kegg-bin/cut_sequence_genes.pl?FROM=5092368&TO=5094223&VECTOR=1&ORG=ece&CHR=cx
Since IclR is naturally made in E. coli, we did not need to do any codon optimization, and the start and stop codons were already included.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]