Difference between revisions of "Part:BBa K399001:Design"

Revision as of 01:50, 12 March 2011

mStrawberry

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 352
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 352
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 352
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 352
1000
COMPATIBLE WITH RFC[1000]

Design Notes

mStrawberry contains a PstI site. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI. Digest the upstream sequence at EcoRI and Spe1.

Emission Spectrum

Characteristic emission spectrum for mStrawberry, showing its percent increase in fluorescence over that of DH5 alhpa cells at specific wavelengths. As expected, peak emission occurs at 600nm [1].

Source

mStrawberry was developed by Roger Tsien¹

References

1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572

@@ Line 13: / Line 13: @@
 [[Image:MStrawberry_graph.jpg|600px|center]]
-Characteristic emission spectrum for mStrawberry, showing its percent increase in fluorescence over that of DH5 alhpa cells at specific wavelengths.  As expected, peak emission occurs at 600nm.
+Characteristic emission spectrum for mStrawberry, showing its percent increase in fluorescence over that of DH5 alhpa cells at specific wavelengths.  As expected, peak emission occurs at 600nm [1].
 ===Source===