Difference between revisions of "Part:BBa K176011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | |||
| + | Primers: | ||
| + | Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. | ||
| + | Sequences provided by Gingko BioWorks. | ||
| + | Fwd: GCTTCTAGAGATACATGAAGATGCACTTttgacggctagctcagtcctagg | ||
| + | |||
| + | Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg | ||
===Source=== | ===Source=== | ||
Latest revision as of 03:49, 30 December 2010
pCon 1.00(J23100)->RBS+GFP+T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
Design Notes
Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.
Fwd: GCTTCTAGAGATACATGAAGATGCACTTttgacggctagctcagtcctagg
Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg
Source
J23100 I13504