Difference between revisions of "Part:BBa J04430:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | Primers: | ||
| + | Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. | ||
| + | Sequences provided by Gingko BioWorks. | ||
| + | Fwd: GCTTCTAGAGATACATGAAGATGCACTTcaatacgcaaaccgcctctccc | ||
| + | Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg | ||
===Source=== | ===Source=== | ||
Latest revision as of 03:46, 30 December 2010
GFP coding device switched on by IPTG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870
Design Notes
Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.
Fwd: GCTTCTAGAGATACATGAAGATGCACTTcaatacgcaaaccgcctctccc
Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg
Source
Davidson Synth-Aces