Difference between revisions of "Part:BBa I13521:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_I13521 short</partinfo> | <partinfo>BBa_I13521 short</partinfo> | ||
| Line 8: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| + | Primers: | ||
| + | Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. | ||
| + | Sequences provided by Gingko BioWorks. | ||
| + | Fwd: GCTTCTAGAGATACATGAACATGCAATACtccctatcagtgatagagattgacatcc | ||
| + | Rv: CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg | ||
===Source=== | ===Source=== | ||
Revision as of 03:44, 30 December 2010
Ptet mRFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 635
Illegal AgeI site found at 747 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.
Fwd: GCTTCTAGAGATACATGAACATGCAATACtccctatcagtgatagagattgacatcc
Rv: CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg