Difference between revisions of "Part:BBa J04450:Design"
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Primers: | Primers: | ||
| − | Note: EcoRI sites removed. Must use XP. | + | Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. |
Sequences provided by Gingko BioWorks. | Sequences provided by Gingko BioWorks. | ||
Latest revision as of 03:43, 30 December 2010
RFP Coding Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.
fwd:GCTTCTAGAGATACATGAACATGCAATACcaatacgcaaaccgcctctccc
rv:CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg
Source
Davidson Synth-Aces