Difference between revisions of "Part:BBa J04450:Design"

(Design Notes)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J04450 short</partinfo>
 
<partinfo>BBa_J04450 short</partinfo>
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===Design Notes===
 
===Design Notes===
  
 +
Primers:
 +
Note: EcoRI sites removed. Must use XP.
 +
Sequences provided by Gingko BioWorks.
  
 
+
fwd:GCTTCTAGAGATACATGAACATGCAATACcaatacgcaaaccgcctctccc
 +
rv:CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg
  
 
===Source===
 
===Source===

Revision as of 03:42, 30 December 2010

RFP Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Sequences provided by Gingko BioWorks.

fwd:GCTTCTAGAGATACATGAACATGCAATACcaatacgcaaaccgcctctccc rv:CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg

Source

Davidson Synth-Aces

References