Difference between revisions of "Part:BBa J70657:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Made from J70610 using the following inserts | + | Made from J70610 using the following inserts and BsaXI digestion |
Latest revision as of 23:40, 20 December 2010
N-terminal 6 his fusion test for GFP J70610w604 inserts
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 863
Design Notes
Made from J70610 using the following inserts and BsaXI digestion
J70604f fromJ70610
5'- AGG AGA AAT ACT AGA TGC ATC ATC ATC ATC ATC ACA GCA GCG GCC GTA -3'
J70604r fromJ70610
5'- GGC CGC TGC TGT GAT GAT GAT GAT GAT GCA TCT AGT ATT TCT CCT CTT -3'
Source
Made using bioscaffolds