Difference between revisions of "Part:BBa J70602:Design"

 
(→‎Design Notes)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70602 short</partinfo>
 
<partinfo>BBa_J70602 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
designed
+
Primer Design Notes:
  
 +
Forward primer: gtttcttcgaattcgcggccgcttctagatgaagattctggtcattggtgctgg
 +
l:54
 +
P(g/b):g
 +
Ho(max#):-109.4
 +
Het(max#):-115.82
 +
Tm:69.6
 +
GC(%):50
  
 +
Reverse primer: gtttcttcctgcagcggccgctactagtattattaagcgctgcaaagcataacgcag
 +
l:57
 +
P(g/b):b
 +
Ho(max#):-115.82
 +
Het(max#):-115.82
 +
Tm:69.8
 +
GC(%):49.1
  
 
===Source===
 
===Source===

Latest revision as of 17:39, 19 November 2010

VioD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 928
    Illegal SapI.rc site found at 1003


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgaagattctggtcattggtgctgg l:54 P(g/b):g Ho(max#):-109.4 Het(max#):-115.82 Tm:69.6 GC(%):50

Reverse primer: gtttcttcctgcagcggccgctactagtattattaagcgctgcaaagcataacgcag l:57 P(g/b):b Ho(max#):-115.82 Het(max#):-115.82 Tm:69.8 GC(%):49.1

Source

designed

References