Difference between revisions of "Part:BBa J70601:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70601 short</partinfo>
 
<partinfo>BBa_J70601 short</partinfo>
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===Design Notes===
 
===Design Notes===
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designed
+
Primer Design Notes:
  
 +
Forward primer: gtttcttcgaattcgcggccgcttctagatgaaacgtgcgattatcgttggtgg
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l: 54
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P(g/b): g
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Ho(max#):-112.47
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Het(max#):-112.88
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Tm: 69.7
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GC(%): 50
  
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Reverse primer: gtttcttcctgcagcggccgctactagtattattaattcacgcgaccaatcttgtacc
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l: 58
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P(g/b): g
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Ho(max#): -112.8
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Het(max#):-112.88
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Tm: 68.5
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GC(%): 46.6
  
 
===Source===
 
===Source===

Latest revision as of 17:38, 19 November 2010

VioC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgaaacgtgcgattatcgttggtgg l: 54 P(g/b): g Ho(max#):-112.47 Het(max#):-112.88 Tm: 69.7 GC(%): 50

Reverse primer: gtttcttcctgcagcggccgctactagtattattaattcacgcgaccaatcttgtacc l: 58 P(g/b): g Ho(max#): -112.8 Het(max#):-112.88 Tm: 68.5 GC(%): 46.6

Source

designed

References