Difference between revisions of "Part:BBa J70599:Design"

 
(→‎Design Notes)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70599 short</partinfo>
 
<partinfo>BBa_J70599 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
designed
+
Primer Design Notes:
  
 +
Forward primer: gtttcttcgaattcgcggccgcttctagatgaaacattcttccgatatctgcattgttg
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l: 59
 +
P(g/b): g
 +
Ho(max#): -116.59
 +
Het(max#): -116.59
 +
Tm: 68.3
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GC(%): 44.1
  
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Reverse primer: gtttcttcctgcagcggccgctactagtattattacgcggcgatacgctgcaac
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l: 54
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P(g/b): b
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Ho(max#): -112.7
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Het(max#): -116.59
 +
Tm: 70.7
 +
GC(%): 53.7
  
 
===Source===
 
===Source===

Latest revision as of 17:36, 19 November 2010

VioA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1012
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgaaacattcttccgatatctgcattgttg l: 59 P(g/b): g Ho(max#): -116.59 Het(max#): -116.59 Tm: 68.3 GC(%): 44.1

Reverse primer: gtttcttcctgcagcggccgctactagtattattacgcggcgatacgctgcaac l: 54 P(g/b): b Ho(max#): -112.7 Het(max#): -116.59 Tm: 70.7 GC(%): 53.7

Source

designed

References