Difference between revisions of "Part:BBa J70028"
 
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There is a definite mutation at base 286 in the J70025 sequence.  Actual construct is tcccggtg instead of tcccagtg.  It is not clear whether this mutation is in part J70025 or not.
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There is a definite mutation at base 286 in the J70025 sequence.  Actual construct is tcccggtg instead of tcccagtg.  It is not clear whether this mutation is in part J70025 or not. This mutation introduces a serine -> glycine substitution in the leader peptide.  Probably insignificant.
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Latest revision as of 17:05, 17 November 2010

tetM tetracycline resistance cassette

J70025 (pBOT1 tetM promoter upstream region) + J70024 (recoded tetM gene from Geneart plasmid construction)


There is a definite mutation at base 286 in the J70025 sequence. Actual construct is tcccggtg instead of tcccagtg. It is not clear whether this mutation is in part J70025 or not. This mutation introduces a serine -> glycine substitution in the leader peptide. Probably insignificant.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]