Difference between revisions of "Part:BBa K392036"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K392036 short</partinfo>
 
<partinfo>BBa_K392036 short</partinfo>
  
 
Yeast expression device containing C. fermi endoglucanase CenA fused with cellulose-binding motif CM10; engineered secretion tag attached upstream to enable secretion of fusion protein.
 
Yeast expression device containing C. fermi endoglucanase CenA fused with cellulose-binding motif CM10; engineered secretion tag attached upstream to enable secretion of fusion protein.
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See experience page for experimental data.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:40, 8 November 2010

endoglucanase/CBM secretion testing construct for yeast

Yeast expression device containing C. fermi endoglucanase CenA fused with cellulose-binding motif CM10; engineered secretion tag attached upstream to enable secretion of fusion protein.

See experience page for experimental data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1604
    Illegal NotI site found at 2748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2661
    Illegal BamHI site found at 1797
    Illegal XhoI site found at 2159
    Illegal XhoI site found at 2408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal NgoMIV site found at 1939
    Illegal NgoMIV site found at 2864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830
    Illegal BsaI site found at 1843