Difference between revisions of "Part:BBa K313007"

(→‎verificatino experiment)
(→‎verificatino experiment)
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=== verificatino experiment ===
 
=== verificatino experiment ===
  
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The part is ligated with inverted pBAD promoter(BBa_K???).
+
The part is ligated with inverted pBAD promoter(BBa_K???) and the plasmid was transformed into JM109.
−
 
+
−
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq  Location sequence] assay page for detail.
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</gallery>
 
</gallery>
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Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.
 +
 +
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq  Location sequence] assay page for detail.
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:44, 7 November 2010

gfp generator in the reverse direction

This part is a gfp generator in reverse direction. It contains terminator. It is basically the reversed version of BBa_E0840.


verificatino experiment

The part is ligated with inverted pBAD promoter(BBa_K???) and the plasmid was transformed into JM109.


  • The result of gfp expression assay

Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq Location sequence] assay page for detail.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 209