Difference between revisions of "Part:BBa K313003"
 
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Reverse reporter(gfp and double terminator in the reverse dircion).  
 
Reverse reporter(gfp and double terminator in the reverse dircion).  
  
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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq  Location sequence] assay page.<br/>
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Please see BBa_313007 for experimental verification of this part.
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===veficication experimet===
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<gallery>
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Image:Gfp.png|The result of gfp expression assay
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</gallery>
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Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.
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Plasmid was transformed into JM109.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:43, 7 November 2010

terminator(reverse direction) and gfp (reverse direction)

Reverse reporter(gfp and double terminator in the reverse dircion).

Please see BBa_313007 for experimental verification of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 209