Difference between revisions of "Part:BBa K313003"

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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq  Location sequence] assay page.<br/>
 
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq  Location sequence] assay page.<br/>
  
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==Figure==
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===veficication experimet===
 
<gallery>
 
<gallery>
 
Image:Gfp.png|The result of gfp expression assay
 
Image:Gfp.png|The result of gfp expression assay

Revision as of 20:36, 7 November 2010

terminator(reverse direction) and gfp (reverse direction)

Reverse reporter(gfp and double terminator in the reverse dircion).

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq Location sequence] assay page.

veficication experimet

  • The result of gfp expression assay

Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.

Plasmid was transformed into JM109.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 209