Difference between revisions of "Part:BBa K313003"

Revision as of 20:35, 7 November 2010

terminator(reverse direction) and gfp (reverse direction)

Reverse reporter(gfp and double terminator in the reverse dircion).

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq Location sequence] assay page.

Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.

Plasmid was transformed into JM109.

Sequence and Features

Assembly Compatibility:

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-Transcription of gfp reverse from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.
+Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.
 Plasmid was transformed into JM109.