Difference between revisions of "Part:BBa R0061:Experience"
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*The results from iGEM Tokyo Tech 2010 (above in this page) shows about 1/3 repression also using '''log phase''' cells. | *The results from iGEM Tokyo Tech 2010 (above in this page) shows about 1/3 repression also using '''log phase''' cells. | ||
*Cox ''et al.'' [2] reported that the "Plux repression promoter"' does not work. This paper measured the cells in a '''stationary phase''' (cultured at 25 ºC for 18 h) as our team did (at 37 ºC for 12 h).<br><br> | *Cox ''et al.'' [2] reported that the "Plux repression promoter"' does not work. This paper measured the cells in a '''stationary phase''' (cultured at 25 ºC for 18 h) as our team did (at 37 ºC for 12 h).<br><br> | ||
− | *From these information, we believe that the reason why we couldn't observe the repression is because we performed the experiment using '''stationary phase''' cells, not log phase cells. | + | *From these information, we believe that the reason why we couldn't observe the repression is because we performed the experiment using '''stationary phase''' cells, not log phase cells.<Br clear="all"> |
− | <Br clear="all"> | + | |
=====Reference===== | =====Reference===== | ||
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications. You can add or remove lines as needed --> | <!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications. You can add or remove lines as needed --> |
Revision as of 07:27, 7 November 2010
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_R0061
User Reviews
UNIQ974085d748b5c81a-partinfo-00000000-QINU
No review score entered. iGEM Tokyo_Tech 2010 |
In order to characterize R0061, Plux repression promoter, we constructed K395101 combining R0061 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) on pSB6A1 and used a fusion of PlacIq (I14032) to gfp (K121013) as a positive control and used promoterless gfp (K121013) as a negative control. |
iGEM Chiba 2010 |
iGEM Chiba 2010
DetailsMaterials & Methods
Results![]() Figure. Fluorescence of GFP under lux repression promoter with/without AHL. p.c. stands for positive control, n.c. stands for negative control. All biobricks are co-transformed with K396011 (constitutive luxR generator). Bar heights are normalized to OD600 and represent the averages of three replicates; error bars, standard deviations.
Reference<biblio>
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UNIQ974085d748b5c81a-partinfo-00000003-QINU