Difference between revisions of "Part:BBa K358021:Experience"
(→Characterization of lytic activity with time) |
|||
| Line 10: | Line 10: | ||
# Incubate the cultures at 30 degrees and measure OD550 of them. | # Incubate the cultures at 30 degrees and measure OD550 of them. | ||
| − | [[Image: | + | [[Image:KyotoL003.png|700px|thumb|center|Fig.1 The result of characterization of lysisbox with time.]] |
As Fig.1 shows, we couldn't observe clearly the function of S<sub>ΔTMD1</sub> as the anti-killer gene against λ lysis cassette. | As Fig.1 shows, we couldn't observe clearly the function of S<sub>ΔTMD1</sub> as the anti-killer gene against λ lysis cassette. | ||
Revision as of 00:13, 7 November 2010
Applications of BBa_K358021
Characterization of lytic activity with time
To characterize the anti-killer gene and if our lysisbox would work or not as we expected, we focused on when cell lysis occurs after induction by IPTG. We did the experiment as this protocol, and we got the following data. Protocol
- Pour 3mL of supplemented M9 medium to a Falcon tube.
- Pick out a colony on the plate of E.coli and put into the Falcon tube.
- Grow it at 30 degrees for 16h.
- Dilute it 50 folds with the same media and incubate until OD550 is about 0.15.
- Ditribute 3ml of the culture to falcon tubes and add certain amount of IPTG.
- Incubate the cultures at 30 degrees and measure OD550 of them.
As Fig.1 shows, we couldn't observe clearly the function of SΔTMD1 as the anti-killer gene against λ lysis cassette.
However, after some discussion, RPU of constitutive promoter wasn't correct and it was too weak. So, we re-constructed lysisbox -type2- (ver.2).
User Reviews
UNIQ087a110403271a24-partinfo-00000000-QINU UNIQ087a110403271a24-partinfo-00000001-QINU