Difference between revisions of "Part:BBa K419015:Experience"

Latest revision as of 17:40, 6 November 2010

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Please enter how you used this part and how it worked out.

Applications of BBa_K419015

Verification of the Part BBa_K419015

The Lux R gene with ribosome binding site (RBS) DNA was amplified from the part BBa_C0062. The Lux R PCR products (756 bp) were digested with EcoR I and Pst I, then ligated with the EcoR I-Pst I treated pSB1C3. After E. coli transformation and plasmid preparation, the part BBa_K419015 for sensing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the Lux R DNA fragment. The sensing system composite was transformed into E. coli for further experiments.

User Reviews

UNIQc14d21681637bba6-partinfo-00000000-QINU UNIQc14d21681637bba6-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K419015===
+'''Verification of the Part BBa_K419015'''
+The Lux R gene with ribosome binding site (RBS) DNA was amplified from the part BBa_C0062. The Lux R PCR products (756 bp) were digested with EcoR I and Pst I, then ligated with the EcoR I-Pst I treated pSB1C3. After E. coli transformation and plasmid preparation, the part BBa_K419015 for sensing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the Lux R DNA fragment. The sensing system composite was transformed into E. coli for further experiments.
+[[image:photo14.jpg]]
 ===User Reviews===