Difference between revisions of "Part:BBa K419016:Experience"

Latest revision as of 17:36, 6 November 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K419016

Verification of the Part BBa_K419016

The lysis device DNA which contained holing and lysozyme genes was amplified from the part BBa_K112021. The lysis device PCR products (981 bp) were digested with EcoR I and Pst I and ligated with the EcoR I-Pst I treated pSB1C3. After transformation and plasmid preparation, the part BBa_k419016 for releasing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the lysis device DNA fragment from two clones. The releasing system composite was transformed into E. coli for further experiment.

User Reviews

UNIQa210e8422685c92f-partinfo-00000000-QINU UNIQa210e8422685c92f-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K419016===
+'''Verification of the Part BBa_K419016'''
+[[image:photo13.jpg]]
+The lysis device DNA which contained holing and lysozyme genes was amplified from the part BBa_K112021. The lysis device PCR products (981 bp) were digested with EcoR I and Pst I and ligated with the EcoR I-Pst I treated pSB1C3. After transformation and plasmid preparation, the part BBa_k419016 for releasing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the lysis device DNA fragment from two clones. The releasing system composite was transformed into E. coli for further experiment.
 ===User Reviews===