Difference between revisions of "Part:BBa K419012:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K419012=== | ===Applications of BBa_K419012=== | ||
| + | '''The Part BBa_k419012 (constitutive promoter-CrtEBIY)Verification of the Part BBa_k419012''' | ||
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| + | We use constitutive promoter to drive the CrtEBIY composite for beta-carotene synthesis. The CrtEBIY composite (BBa_K274210) was obtained from the distribution part of iGEM 2009 Cambridge and digested with restriction enzymes, EcoR I and Pst I, then ligated to EcoR I and Pst I sites of pSB1C3, the iGEM 2010 standard backbone. We verified our composite for synthesis system by EcoRI and PstI digestion and the agarose gel electrophoresis result was shown. The arrow (red) indicates the CrtEBIY with constitutive promoter (R001) DNA fragment. The synthesis system composite was transformed into DH5-alpha E. coli for further experiment. | ||
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| + | [[Image:photo2.jpg]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:19, 6 November 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K419012
The Part BBa_k419012 (constitutive promoter-CrtEBIY)Verification of the Part BBa_k419012
We use constitutive promoter to drive the CrtEBIY composite for beta-carotene synthesis. The CrtEBIY composite (BBa_K274210) was obtained from the distribution part of iGEM 2009 Cambridge and digested with restriction enzymes, EcoR I and Pst I, then ligated to EcoR I and Pst I sites of pSB1C3, the iGEM 2010 standard backbone. We verified our composite for synthesis system by EcoRI and PstI digestion and the agarose gel electrophoresis result was shown. The arrow (red) indicates the CrtEBIY with constitutive promoter (R001) DNA fragment. The synthesis system composite was transformed into DH5-alpha E. coli for further experiment.
User Reviews
UNIQ1a3cda90f7d2ad7d-partinfo-00000000-QINU UNIQ1a3cda90f7d2ad7d-partinfo-00000001-QINU