Difference between revisions of "Part:BBa K358021:Experience"

(→‎Applications of BBa_K358021)
Line 1: Line 1:
−
__NOTOC__
 
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
−
how you used this part and how it worked out.
 
−
 
 
===Applications of BBa_K358021===
 
===Applications of BBa_K358021===
 
====Characterization of lytic activity with time====  
 
====Characterization of lytic activity with time====  

Revision as of 10:16, 6 November 2010

Applications of BBa_K358021

Characterization of lytic activity with time

To characterize the lytic activity of our lysisbox if it would work or not as we expected, we focused on when cell lysis occurs after induction by IPTG. We did the experiment as this protocol, and we got the following data. Protocol

  1. Pour 3mL of supplemented M9 medium to a Falcon tube.
  2. Pick out a colony on the plate of E.coli and put into the Falcon tube.
  3. Grow it at 30 degreees for 16h.
  4. Dilute it 50 folds with the same media and incubate until OD550 is about 0.15.
  5. Ditribute 3ml of the culture to falcon tubes and add certain amount of IPTG.
  6. Incubate the cultures at 30 degrees and measure OD550 of them.

User Reviews

UNIQ2be78bc282d14bf8-partinfo-00000000-QINU UNIQ2be78bc282d14bf8-partinfo-00000001-QINU