Difference between revisions of "Part:BBa K345669:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K345669 short</partinfo> | <partinfo>BBa_K345669 short</partinfo> | ||
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The EcoRI and PstI restriction sites are inactivated by mutagenesis so as not to create two sets of these restriction sites. A BsiWI restriction site is created between the XbaI and SpeI restriction sites for ligation independent cloning. | The EcoRI and PstI restriction sites are inactivated by mutagenesis so as not to create two sets of these restriction sites. A BsiWI restriction site is created between the XbaI and SpeI restriction sites for ligation independent cloning. | ||
| − | + | [[Image:BBa_K345669(pSB1C4) plasmid map.jpg]] | |
===Source=== | ===Source=== | ||
Latest revision as of 23:17, 5 November 2010
psb1c3 -> BsiWI
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NotI site found at 2057 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XhoI site found at 1035
Illegal XhoI site found at 1927 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
The EcoRI and PstI restriction sites are inactivated by mutagenesis so as not to create two sets of these restriction sites. A BsiWI restriction site is created between the XbaI and SpeI restriction sites for ligation independent cloning.
_plasmid_map.jpg)
Source
pSB1C3