Difference between revisions of "Part:BBa K313018"

Revision as of 20:59, 5 November 2010

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B1006. If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator. The difference between this part and BBa K313017 is the number of the terminator.

verification experiments

This part is ligated with loxP-gfp-loxP sequence and transformed into E.coli. By incubating E.coli, then extracting plasmids, and sequencing them, it was proved that cre generated by BBa_K313018 is able to catalyze site specific recombination reaction.

Please see our[http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page for detail.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 525
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 211
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 The difference between this part and BBa K313017 is the number of the terminator.
-Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.
+===verification experiments===
+This part is ligated with loxP-gfp-loxP sequence and transformed into E.coli. By incubating E.coli, then extracting plasmids, and sequencing them, it was proved that cre generated by BBa_K313018 is able to catalyze site specific recombination reaction.
+Please see our[http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page for detail.
 <!-- Add more about the biology of this part here