Difference between revisions of "Part:BBa K341002"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K341002 short</partinfo>
 
<partinfo>BBa_K341002 short</partinfo>
Line 11: Line 10:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K341002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K341002 SequenceAndFeatures</partinfo>
 +
 +
 +
===Safety===
 +
This part is for research use only, no special safety issues need to be mentioned.
 +
 +
===References===
 +
Thomas E. Kuhlman and Edward C. Cox:Site-specific chromosomal integration of large synthetic constructs,Nucleic Acids Research, 2009, 1–10
 +
 +
===Acknowledgement===
 +
The physical DNA is from Vecter "pTKs-CS" which is generously provided by Thomas E. Kuhlman and Edward C. Cox of the Department of Molecular Biology, Princeton University.
 +
 +
We are grateful to Guoqiang Chen, Zhao Wang,Yinghua Chen and Liping Xie of School of Life Sciences, Tsinghua University for assistance in the design, construction and characterization of this part.
 +
 +
  
  

Latest revision as of 20:07, 5 November 2010

promoter+tet-SDS

tet-SDS gene together with Lac-promoter working as a reporter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1147
  • 1000
    COMPATIBLE WITH RFC[1000]


Safety

This part is for research use only, no special safety issues need to be mentioned.

References

Thomas E. Kuhlman and Edward C. Cox:Site-specific chromosomal integration of large synthetic constructs,Nucleic Acids Research, 2009, 1–10

Acknowledgement

The physical DNA is from Vecter "pTKs-CS" which is generously provided by Thomas E. Kuhlman and Edward C. Cox of the Department of Molecular Biology, Princeton University.

We are grateful to Guoqiang Chen, Zhao Wang,Yinghua Chen and Liping Xie of School of Life Sciences, Tsinghua University for assistance in the design, construction and characterization of this part.