Difference between revisions of "Part:BBa K353009:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K353009=== | ===Applications of BBa_K353009=== | ||
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| + | This part consisted of BBa_F2620, a promoter recognizing AHL, coupled with a custom-designed sRNA (sRNA1) that binds to the RSID and RBS from BBa_K353002, inhibiting the production of GFP. This part was tested with BBa_K2353002 and characterized on plasmid 1C3 in BW27783 cells. Cells were initially incubated with L-arabinose at the concentrations (%) shown in the legend. Twenty microliters of 10mg/mL AHL (213.2 MW) was added to the plate reader at time, t=0. GFP fluorescence data was collected on a plate reader for two hours every thirteen minutes and is shown in the graph below. As can be seen, addition of AHL to the BW cells does, in fact, seem to downregulate the fluorescence and consequently, functional GFP output | ||
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| + | [[Image:L-Arabinose induced AHL addition.jpg]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 01:36, 5 November 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K353009
This part consisted of BBa_F2620, a promoter recognizing AHL, coupled with a custom-designed sRNA (sRNA1) that binds to the RSID and RBS from BBa_K353002, inhibiting the production of GFP. This part was tested with BBa_K2353002 and characterized on plasmid 1C3 in BW27783 cells. Cells were initially incubated with L-arabinose at the concentrations (%) shown in the legend. Twenty microliters of 10mg/mL AHL (213.2 MW) was added to the plate reader at time, t=0. GFP fluorescence data was collected on a plate reader for two hours every thirteen minutes and is shown in the graph below. As can be seen, addition of AHL to the BW cells does, in fact, seem to downregulate the fluorescence and consequently, functional GFP output
User Reviews
UNIQ8e327f0b840fafd3-partinfo-00000000-QINU UNIQ8e327f0b840fafd3-partinfo-00000001-QINU