Difference between revisions of "Part:BBa K313000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
T7 RNA polymerase induces the expression of gfp from this part. | T7 RNA polymerase induces the expression of gfp from this part. | ||
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=== Verification Experiment === | === Verification Experiment === | ||
| + | Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page for detail. | ||
Plasmid containing this part was transformed to Rosetta (DE3) plysS strain which have T7 polymerase under the control of Lac promoter. | Plasmid containing this part was transformed to Rosetta (DE3) plysS strain which have T7 polymerase under the control of Lac promoter. | ||
Revision as of 13:14, 2 November 2010
gfp generator with T7 promoter
gfp generator with T7 promoter
Usage and Biology
T7 RNA polymerase induces the expression of gfp from this part.
Verification Experiment
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page for detail.
Plasmid containing this part was transformed to Rosetta (DE3) plysS strain which have T7 polymerase under the control of Lac promoter. When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM Fluorescence was measured and the results are shown below:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 719