Difference between revisions of "Part:BBa K358022:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K358022===
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====Experiment 1:Check the role of TMD1 of S gene====
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[[image:GoalC fig3-2-2.png|450px|right|thumb|Fig.1.the comparison between lacL and lacL&Delta;. The black lines on bars' edges are error bar.]]
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Then, in order to check if TMD1 of S gene is essential for this killer gene, we measured the cell number of lacL&Delta;, which does not have TMD1 of S gene and that is, encodes S<sub>&Delta;TMD1</sub> and that of lysis cassette.
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This result is below (Fig.1).
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From this result, after IPTG was added, the number of lacL&Delta; was not decreasing, while the number of lacL&Delta; was decresing.
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It proved that S<sub>&Delta;TMD1</sub> cannot function as killer gene and TMD1 is essential for this killer gene, lysis cassette.
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In addition, in the medium without IPTG, the number of lacL&Delta; is larger than that of lacL&Delta;. This supports a report <sup>[[#RefL001|[1]]]</sup>, which says that more short proteins are expressed than the ones that are long.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 15:12, 31 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Experiment 1:Check the role of TMD1 of S gene

Fig.1.the comparison between lacL and lacLΔ. The black lines on bars' edges are error bar.

Then, in order to check if TMD1 of S gene is essential for this killer gene, we measured the cell number of lacLΔ, which does not have TMD1 of S gene and that is, encodes SΔTMD1 and that of lysis cassette. This result is below (Fig.1). From this result, after IPTG was added, the number of lacLΔ was not decreasing, while the number of lacLΔ was decresing. It proved that SΔTMD1 cannot function as killer gene and TMD1 is essential for this killer gene, lysis cassette. In addition, in the medium without IPTG, the number of lacLΔ is larger than that of lacLΔ. This supports a report [1], which says that more short proteins are expressed than the ones that are long.

User Reviews

UNIQ97cc4ac3ba014392-partinfo-00000000-QINU UNIQ97cc4ac3ba014392-partinfo-00000001-QINU