Difference between revisions of "Part:BBa K371053"

 
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<partinfo>BBa_K371053 short</partinfo>
 
<partinfo>BBa_K371053 short</partinfo>
  
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This part locate in BBa_K371051, which is very like pSB1C3 with only a single site nonsense mutation between terminator 0 and chloramphenicol resistance gene. The function of this gene is to convert the any RFC53 part to RFC 10 part without leaving any trace. User will find it is very convenient to make fusion protein with this noval system.
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This part locate in BBa_K371051, which is very like pSB1C3 with only a single site nonsense mutation between terminator 0 and chloramphenicol resistance gene. This part is used to make a plasmid compatible to RFC 53 in addition to RFC 10. So plasmid with this part complies with both 10 and 53 assembly standard. Then protein fusion can easily be done in this new plasmid.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:34, 31 October 2010

theRFC 10 and RFC53 interface converter

This part locate in BBa_K371051, which is very like pSB1C3 with only a single site nonsense mutation between terminator 0 and chloramphenicol resistance gene. This part is used to make a plasmid compatible to RFC 53 in addition to RFC 10. So plasmid with this part complies with both 10 and 53 assembly standard. Then protein fusion can easily be done in this new plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 14
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3